1/16/2023 0 Comments Nod scid mouse![]() ![]() Philadelphia (PA): AACR Cancer Res 2010 70(8 Suppl):Abstract nr 393. Anesthetized, plateletdepleted NOD/SCID mice were subsequently infused with 111 Indium radiolabelled human platelets via an exposed femoral vein and the responses to intravenous injection of platelet agonists recorded as changes in plateletassociated counts via external 1cm scintillation counters positioned over the thoracic region. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research 2010 Apr 17-21 Washington, DC. Moreover, multiple defects in innate and adaptive immunity are seen in these mice, making them better recipients for human hematopoietic stem cell and human solid tumor transplantation 18. The NOD/SCID mice do not develop diabetes because they lose functional T lymphocytes. As the least leaky, most immunocompromised hairless model available, the SHrN™ model will become a valuable enhancement to oncology and immunology research, with particular benefit to imaging technology studies.Ĭitation Format. The NOD/SCID mice were established by crossbreeding NOD and SCID mice. The immunologic characteristics of this model are also described, which include B and T cell deficiency, a functional deficit of NK cells, and decreased macrophage and complement function. Littermates identified to have a genetic background 99% concordant to the NOD mouse were sibling-mated to produce F1 breeders homozygous for both Prkdc scid and Hr hr mutations. In each backcross generation, a total of 560 SNPs was measured on all offspring homozygous for Prkdc scid and heterozygous for Hr hr to select the most optimal breeders with the highest percentage of NOD genetic background. SNP genotypes were determined using the Fluidigm Biomark™ High Throughput SNP Genotyping Platform. A combination of single nucleotide polymorphism (SNP) allelic discrimination and quantitative PCR (QPCR) analysis was employed to genotype offspring for the Prkdc scid and Hr hr mutations. Herein we describe the breeding program utilized to generate the SHrN™ model using marker assisted backcrossing methodology. We have transferred the hairless mutation (Hr hr) from the outbred HsdOla:MF1-Hr hr to the NOD.CB17-Prkdc scid/NCrHsd mouse to generate the inbred hairless NOD.Cg-Prkdc scid Hr hr/NCrHsd mouse, or commonly named scid hairless NOD (SHrN™). The test was applied to NOD/SCID mice transplanted. However, shaving animals does not prevent autofluorescence of hair follicles while imaging scid mice. Serial dilutions of defined numbers of human cells in mouse cells served to construct calibration curves. As scid mice are haired, it is often necessary to shave these mice to facilitate tumor observation and evaluation. It has proven to be particularly useful in elucidating mechanisms of spontaneous lymphoma and thymic tumors, prostate cancer research, and the engraftment of hematopoietic stem cells. The non-obese diabetic (NOD) mouse carrying the Prkdc scid mutation (NOD scid mouse) is an established xenograft model for humanization, oncology, and immunology research. The SHrN™ scid hairless NOD mouse was developed to provide a hairless triple-immunodeficient mouse model to the research community. ![]()
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